The last two STL Northern League teams left in the competition were drawn together on Monday lunchtime.
The sides have met twice previously in the Vase, with Whitley coming out on top on both occasions, firstly during their 2001-02 Vase winning season when a 3-2 win at Dunston saw Whitley into the second round and then the following year when, again at Dunston, Whitley were 2-0 winners in a third round game.
When the teams met earlier this season at Hillheads, Whitley gained a hard fought 2-1 victory.
However, Dunston, like Whitley, are on an excellent run of forms.
The other three games are Poole Town v Torpoint Athletic; Coalville Town v Leiston and King’s Lynn v Rye United.
The ties are on Saturday, March 5.